Perspectives in Hydrophobic Interaction Temperature- Responsive Liquid Chromatography (TRLC)
نویسندگان
چکیده
Temperature-responsive liquid chromatography (TRLC) is an emerging green high performance (HPLC) mode allowing reversed phase-type separations while necessitating only water as the mobile phase. The columns therein are typically packed with silica particles to which stimuli-responsive polymers anchored. In hydrophobic interaction TRLC, such depict a loss of solubility when increasing temperature above characteristic conversion temperature, causing large changes in retention over quite narrow and mild ranges (~5–55 °C). TRLC circumvents concerns about analyte or column degradation that can occur implementing temperatures (>80 °C) on conventional reversed- phase columns. It allows for using often spiked additives used reversed-phase LC. Therefore, this separation greener, cheaper, isocratic analyses under non-denaturing conditions. absence compositional solvent gradients also exploitation combination refractive index detection. Purely aqueous mostly applicable solutes depicting 1 < LogP 5, yet these be expanded through implementation combined organic mobiles phases, preserving temperature-responsive effects. first installment, our recent developments, new possibilities, current limitations use 1-D discussed, described respect fundamentals HPLC.
منابع مشابه
Hydrophobic Interaction Chromatography
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ژورنال
عنوان ژورنال: Lc Gc North America
سال: 2022
ISSN: ['1527-5949']
DOI: https://doi.org/10.56530/lcgc.na.vd2373d8